Journal: Materials Today Bio
Article Title: Metformin glycyrrhetinic acid binary injectable hydrogel for synergistic tumor immunotherapy via spatiotemporal microenvironment remodeling
doi: 10.1016/j.mtbio.2025.102749
Figure Lengend Snippet: In vivo immune activation of Fe 3 O 4 NPs@Met-GA-H. ( A, B ) Immunofluorescence staining of HMGB1 (A, red) and CRT (B, red) in tumor tissues following various treatments. Blue: DAPI; scale bars: 100 μm. (n = 5). ( C, D ) Immunofluorescent staining of CD206 (C, green) and CD86 (D, green) in tumor sections. Blue: DAPI; scale bars: 100 μm. (n = 5). ( E ) Immunofluorescence staining of PD-L1 expression (red) in tumors across treatment groups. Blue: DAPI; scale bars: 100 μm. (n = 5). ( F, G ) Flow cytometry analysis of MHC-II expression (F) and CD80 + +CD86 + populations (G) in DCs from spleens. (n = 5). ( H ) Immunofluorescence co-staining of CD4 (green) and CD8 (red) in tumor tissues. Blue: DAPI; scale bars: 100 μm. (n = 5). Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA. Ns: not significant ( P > 0.05); ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 versus the Ctrl. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H + L) (Cat. No. 111-585-003, dilution: 1:250) was sourced from Jackson ImmunoResearch Inc. Rabbit anti-CD86 antibody (Cat. No. WL05184, dilution: 1:200), rabbit anti-iNOS antibody (Cat. No. WL0992a, dilution: 1:200), rabbit anti-CD206 antibody (Cat. No. WL06177, dilution: 1:200), and fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit IgG (H + L) (Cat. No. WLA032, dilution: 1:200) were purchased from Wanlei Bio-Tech Co., Ltd. Rabbit anti-PD-L1 antibody (Cat. No. bs-1103R, dilution: 1:250) was obtained from Beijing Bioss Biotechnology Co., Ltd. PerCP/Cyanine5.5 anti-mouse CD45 Recombinant Antibody (Cat. No. 157612, dilution: 1:200), PE anti-mouse CD11c Antibody (Cat. No. 117308, dilution: 1:200), APC anti-mouse CD86 Antibody (Cat. No. 159216, dilution: 1:200), FITC anti-mouse CD80 Antibody (Cat. No. 104706, dilution: 1:200), PE/Cyanine7 anti-mouse I-A b Antibody (Cat. No. 116420, dilution: 1:200), Zombie R718TM Fixable Viability Kit (Cat. No. 423116, dilution: 1:200), FITC anti-mouse CD3 Antibody (Cat. No. 100204, dilution: 1:100), PE anti-mouse CD4 Antibody (Cat. No. 100408, dilution: 1:200), APC/Cyanine7 anti-mouse CD8a Antibody (Cat. No. 100714, dilution: 1:200), APC anti-mouse/human CD11b Antibody (Cat. No. 101212, dilution: 1:200), APC/FireTM 750 anti-mouse F4/80 Antibody (Cat. No. 123152, dilution: 1:200), PE/DazzleTM 594 anti-mouse CD206 (MMR) Antibody (Cat. No. 141732, dilution: 1:200), and PE/Cyanine7 anti-mouse CD86 Antibody (Cat. No. 159208, dilution: 1:200) were purchased from BioLegend.
Techniques: In Vivo, Activation Assay, Immunofluorescence, Staining, Expressing, Flow Cytometry